CLSI C61

Determination of Serum Iron, Total Iron-Binding Capacity and Percent Transferrin Saturation, 1st Edition

This document provides methods for determining serum iron and total iron-binding capacity; and describes the measurement of serum iron concentration as well as the determination of the percent saturation of transferrin with iron.

This document is available in electronic format only.

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Chairholder: Onno van Assendelft, MD, PhD

Date of Publication: December 1, 1998

Order Code PDF: CLSI C61AE
ISBN Number: 1-56238-362-0

Order Code Print: print not available

Edition: First

Pages: 60

CLSI C61 Additional Details

If interested in ordering larger quantities of this document in print, please contact us here.

This document was formerly sold under the code H17.

This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid as of September 2016. Because of its value to the laboratory community, it is being retained in CLSI’s library.

CLSI C61 Abstract

This document describes the measurement of serum iron concentration, provides guidelines for the determination of serum total iron-binding capacity, and describes the determination of the percent saturation of transferrin with iron. The methods are linear over a wide range of iron concentrations, interference is negligible, and the precision is adequate. Although the methods, as described, are tedious and demand a large sample size and meticulous processing, they can be (semi)automated and the required amount of sample can be decreased. An example of automation is included. The document also provides reference ranges by age groups, race, and gender for serum iron concentration, total iron-binding capacity, and % transferrin saturation as determined during the U.S. National Health and Nutrition Examination Surveys, 1971-74, 1976-80, and 1988-94.